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canine igg heavy  (Jackson Immuno)


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    Jackson Immuno canine igg heavy
    Canine anti‐IgLON5 antibodies. Immunofluorescence studies: Diluted serum (1:20) from a dog from the epilepsy/dyskinesia group (Golden Retriever, female, 3 years) is incubated with IgLON5‐transfected human embryonic kidney (HEK) cells. Binding of antibodies to cell surfaces is visualized by two different secondary anti‐canine antibodies coupled with immunofluorescence dyes and subsequently overlaid. The results demonstrate that there are canine anti‐IgLON5 <t>IgG</t> antibodies. Endpoint titration gave a titer of 1:40 (not shown). (A) <t>anti‐canine</t> <t>IgG</t> heavy and light chain (a sensitive secondary antibody), coupled with the red Alexa 594 dye. (B) overlay; yellow: double‐stainings. (C) anti‐canine IgG Fc fragment (the more specific secondary antibody), coupled with the green Alexa 488 dye. Bar: 25 μm.
    Canine Igg Heavy, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/canine igg heavy/product/Jackson Immuno
    Average 92 stars, based on 7 article reviews
    canine igg heavy - by Bioz Stars, 2026-03
    92/100 stars

    Images

    1) Product Images from "Investigation of the presence of specific neural antibodies in dogs with epilepsy or dyskinesia using murine and human assays"

    Article Title: Investigation of the presence of specific neural antibodies in dogs with epilepsy or dyskinesia using murine and human assays

    Journal: Journal of Veterinary Internal Medicine

    doi: 10.1111/jvim.16744

    Canine anti‐IgLON5 antibodies. Immunofluorescence studies: Diluted serum (1:20) from a dog from the epilepsy/dyskinesia group (Golden Retriever, female, 3 years) is incubated with IgLON5‐transfected human embryonic kidney (HEK) cells. Binding of antibodies to cell surfaces is visualized by two different secondary anti‐canine antibodies coupled with immunofluorescence dyes and subsequently overlaid. The results demonstrate that there are canine anti‐IgLON5 IgG antibodies. Endpoint titration gave a titer of 1:40 (not shown). (A) anti‐canine IgG heavy and light chain (a sensitive secondary antibody), coupled with the red Alexa 594 dye. (B) overlay; yellow: double‐stainings. (C) anti‐canine IgG Fc fragment (the more specific secondary antibody), coupled with the green Alexa 488 dye. Bar: 25 μm.
    Figure Legend Snippet: Canine anti‐IgLON5 antibodies. Immunofluorescence studies: Diluted serum (1:20) from a dog from the epilepsy/dyskinesia group (Golden Retriever, female, 3 years) is incubated with IgLON5‐transfected human embryonic kidney (HEK) cells. Binding of antibodies to cell surfaces is visualized by two different secondary anti‐canine antibodies coupled with immunofluorescence dyes and subsequently overlaid. The results demonstrate that there are canine anti‐IgLON5 IgG antibodies. Endpoint titration gave a titer of 1:40 (not shown). (A) anti‐canine IgG heavy and light chain (a sensitive secondary antibody), coupled with the red Alexa 594 dye. (B) overlay; yellow: double‐stainings. (C) anti‐canine IgG Fc fragment (the more specific secondary antibody), coupled with the green Alexa 488 dye. Bar: 25 μm.

    Techniques Used: Immunofluorescence, Incubation, Transfection, Binding Assay, Titration



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    Image Search Results


    Canine anti‐IgLON5 antibodies. Immunofluorescence studies: Diluted serum (1:20) from a dog from the epilepsy/dyskinesia group (Golden Retriever, female, 3 years) is incubated with IgLON5‐transfected human embryonic kidney (HEK) cells. Binding of antibodies to cell surfaces is visualized by two different secondary anti‐canine antibodies coupled with immunofluorescence dyes and subsequently overlaid. The results demonstrate that there are canine anti‐IgLON5 IgG antibodies. Endpoint titration gave a titer of 1:40 (not shown). (A) anti‐canine IgG heavy and light chain (a sensitive secondary antibody), coupled with the red Alexa 594 dye. (B) overlay; yellow: double‐stainings. (C) anti‐canine IgG Fc fragment (the more specific secondary antibody), coupled with the green Alexa 488 dye. Bar: 25 μm.

    Journal: Journal of Veterinary Internal Medicine

    Article Title: Investigation of the presence of specific neural antibodies in dogs with epilepsy or dyskinesia using murine and human assays

    doi: 10.1111/jvim.16744

    Figure Lengend Snippet: Canine anti‐IgLON5 antibodies. Immunofluorescence studies: Diluted serum (1:20) from a dog from the epilepsy/dyskinesia group (Golden Retriever, female, 3 years) is incubated with IgLON5‐transfected human embryonic kidney (HEK) cells. Binding of antibodies to cell surfaces is visualized by two different secondary anti‐canine antibodies coupled with immunofluorescence dyes and subsequently overlaid. The results demonstrate that there are canine anti‐IgLON5 IgG antibodies. Endpoint titration gave a titer of 1:40 (not shown). (A) anti‐canine IgG heavy and light chain (a sensitive secondary antibody), coupled with the red Alexa 594 dye. (B) overlay; yellow: double‐stainings. (C) anti‐canine IgG Fc fragment (the more specific secondary antibody), coupled with the green Alexa 488 dye. Bar: 25 μm.

    Article Snippet: Secondary AB was a polyclonal rabbit anti‐dog immunoglobulin‐G (IgG) AB directed against canine IgG heavy and light chains (catalog no. 304‐065‐003; Jackson ImmunoResearch/Dianova) conjugated with red Alexa Fluor 594 used at a dilution of 1:100 and incubated for 30 minutes at room temperature; nuclei were counterstained with Hoechst 33342, 1:10 000.

    Techniques: Immunofluorescence, Incubation, Transfection, Binding Assay, Titration